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Environmental Engineering, Undergraduate

Environmental Engineering,

Subsurface Biosphere Initiative

Lewis Semprini

Distinguished Professor of Environmental Engineering
Chair of the Executive Committee, Subsurface Biosphere Initiative

Curriculum Vita
Journal Articles
Conference Proceedings
Research Reports
Other Publications
Online Tutorials

Lew Semprini

Courses Taught

  • ENVE 554 Groundwater Remediation (link to related online tutorials)
  • ENVE 535 Hazardous Waste Remediation
  • ENVE431/531 Fate and Transport of Organic Chemicals in the Environmentt
  • ENVE 490 Environmental Engineering Capstone Design

Research Projects

Global Transcriptional Responses in Nitrogen Cycling and Nutrient Removal Processes [Slide]

Molecular Biomarkers for Detecting, Monitoring and Quantifying Reductive Microbial Processes

Identifying the Inhibition and Expression of Sentinel Genes of the Bacteria N. Europa Upon Exposure to Metal Oxide Nanoparticles

Provost's Initiative on Subsurface Biosphere Education and Research [Description]

Recent Publications

T.S. Radniecki, C. Gilroy, and L. Semprini. Linking NE1545 expression with cell size changes in Nitrosomonas europaea cells exposed to aromatic hydrocarbons, Chemosphere (2011) 82 514-520. DOI link:

Lauchnor, Ellen G., Tyler S. Radniecki, Lewis Semprini, Inhibition and gene expression of Nitrosomonas europaea biofilms exposed to phenol and toluene, Biotechnology and Bioengineering  (2010) 108 (4) 750–757. DOI link:

Taylor, Anne E., Daniel J. Arp, Peter J. Bottomley, Lewis Semprini, Extending the alkene substrate range of vinyl chloride utilizing Nocardioides sp. strain JS614 with ethene oxide, Appl Microbiol Biotechnol (2010) 87:2293–2302. DOI link:

Radniecki, Tyler S. , Lewis Semprini, Mark E. Dolan, Expression of merA, trxA, amoA, and hao in continuously cultured Nitrosomonas europaea cells exposed to cadmium sulfate additions, Biotechnology and Bioengineering, 104 (5) (2009) 1004-1011. DOI link:

Yu, S. and, L. Semprini, Enhanced reductive dechlorination of PCE DNAPL with TBOS as a slow-release electron donor, J. Hazard. Mater. 167 (2009) 97–104. DOI link:

Radniecki, Tyler S., Lewis Semprini, and Mark E. Dolan, Expression of merA, amoA and hao in Continuously Cultured Nitrosomonas europaea Cells Exposed to Zinc Chloride Additions, Biotechnology and Bioengineering, 102 (2) (2009) 546-553. DOI link:

Semprini, L., Mark E. Dolan, Gary D. Hopkins, and Perry L. McCarty, Bioaugmentation with butane-utilizing microorganisms to promote in situ cometabolic treatment of 1,1,1-trichloroethane and 1,1-dichloroethene, Journal of Contaminant Hydrology 103, (2009) 157-167. DOI link:

Azizian, M.F., S. Behrens, A. Sabalowsky, M.E. Dolan, A.M. Spormann, and L. Semprini, Continuous-flow column study of reductive dehalogenation of PCE upon bioaugmentation with the Evanite enrichment culture, Journal of Contaminant Hydrology 100 (2008) 11–21. DOI link:

Behrens, Sebastian, Mohammad F. Azizian, Paul J. McMurdie, Andrew Sabalowsky, Mark E. Dolan, Lew Semprini, and Alfred M. Spormann, Monitoring Abundance and Expression of "Dehalococcoides" Species Chloroethene-Reductive Dehalogenases in a Tetrachloroethene-Dechlorinating Flow Column, Appl. Envir. Microbiol. (2008) 74: 5695-5703. DOI link:

Kim, Y., J. D. Istok, L. Semprini, Single-well, gas-sparging tests for evaluating the in situ aerobic cometabolism of cis-1,2-dichloroethene and trichloroethene, Chemosphere 71 (2008) 1654–1664. DOI link:

List of All Journal Articles