Lewis Semprini

Environmental Engineering, Undergraduate Environmental Engineering, Graduate Subsurface Biosphere Initiative Western Region Hazardous Substance Research Center Earth's Subsurface Biosphere (ESB) IGERT Program	Lewis Semprini Distinguished Professor of Environmental Engineering Chair of the Executive Committee, Subsurface Biosphere Initiative Curriculum Vita Journal Articles Conference Proceedings Research Reports Other Publications Online Tutorials Contact
Courses Taught ENVE 554 Groundwater Remediation (link to related online tutorials) ENVE 535 Hazardous Waste Remediation ENVE431/531 Fate and Transport of Organic Chemicals in the Environment Research Projects Aerobic (Co)Metabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols [Slide] Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity [Slide] Global Transcriptional Responses in Nitrogen Cycling and Nutrient Removal Processes [Slide] Provost's Initiative on Subsurface Biosphere Education and Research [Description] Recent Publications Yu, S. and, L. Semprini, Enhanced reductive dechlorination of PCE DNAPL with TBOS as a slow-release electron donor, J. Hazard. Mater. 167 (2009) 97–104. DOI link: http://dx.doi.org/10.1016/j.jhazmat.2008.12.087 Radniecki, Tyler S., Lewis Semprini, and Mark E. Dolan, Expression of merA, amoA and hao in Continuously Cultured Nitrosomonas europaea Cells Exposed to Zinc Chloride Additions, Biotechnology and Bioengineering, 102 (2) (2009) 546-553. DOI link: http://dx.doi.org/10.1002/bit.22069 Semprini, L., Mark E. Dolan, Gary D. Hopkins, and Perry L. McCarty, Bioaugmentation with butane-utilizing microorganisms to promote in situ cometabolic treatment of 1,1,1-trichloroethane and 1,1-dichloroethene, Journal of Contaminant Hydrology 103, (2009) 157-167. DOI link: http://dx.doi.org/10.1016/j.jconhyd.2008.10.005 Azizian, M.F., S. Behrens, A. Sabalowsky, M.E. Dolan, A.M. Spormann, and L. Semprini, Continuous-flow column study of reductive dehalogenation of PCE upon bioaugmentation with the Evanite enrichment culture, Journal of Contaminant Hydrology 100 (2008) 11–21. DOI link: http://dx.doi.org/10.1016/j.jconhyd.2008.04.006 Behrens, Sebastian, Mohammad F. Azizian, Paul J. McMurdie, Andrew Sabalowsky, Mark E. Dolan, Lew Semprini, and Alfred M. Spormann, Monitoring Abundance and Expression of "Dehalococcoides" Species Chloroethene-Reductive Dehalogenases in a Tetrachloroethene-Dechlorinating Flow Column, Appl. Envir. Microbiol. 2008 74: 5695-5703. DOI link: http://dx.doi.org/10.1128/AEM.00926-08 Kim, Y., J. D. Istok, L. Semprini, Single-well, gas-sparging tests for evaluating the in situ aerobic cometabolism of cis-1,2-dichloroethene and trichloroethene, Chemosphere 71 (2008) 1654–1664. DOI link: http://dx.doi.org/10.1016/j.chemosphere.2008.01.021 Semprini, L., M. E. Dolan, M. A. Mathias, G. D. Hopkins and P. L. McCarty, Bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane, European Journal of Soil Biology 43, (5-6), 322-327 (2007) (In situ Bioremediation, C/Tur Third European Bioremediation Conference). DOI link: http://dx.doi.org/10.1016/j.ejsobi.2007.03.006 A.E. Taylor, M.E. Dolan, P.J. Bottomley, and L. Semprini. Utilization of Fluoroethene as a Surrogate for Aerobic Vinyl Chloride Degradation. Environ. Sci. Technol., 41 (18) 6378–6383 (2007). DOI link: http://dx.doi.org/10.1021/es0701255 Yu, S. and L. Semprini. Enhanced Reductive Dechlorination of PCE DNAPL with TBOS as a Slow-Release Substrate. In review, Journal of Hazardous Waste. Semprini, L., M. E. Dolan, M. A. Mathias, G. D. Hopkins and P. L. McCarty, Laboratory, field and modeling studies of bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane, Advances in Water Resources, 30 1528-1546 (2007). DOI link: http://dx.doi.org/10.1016/j.advwatres.2006.05.017 Azizian, M., J. D. Istok, L. Semprini, Evaluation of the in-situ aerobic cometabolism of chlorinated ethenes by toluene-utilizing microorganisms using push–pull tests, Journal of Contaminant Hydrology, 90 105–124 (2007). DOI link: http://dx.doi.org/10.1016/j.jconhyd.2006.09.015 Kim, Y., Istok, J. and L. Semprini, Push–pull tests evaluating in situ aerobic cometabolism of ethylene, propylene, and cis-1,2-dichloroethylene, Journal of Contaminant Hydrology, 82:165-181 (2006). DOI link: http://dx.doi.org/10.1016/j.jconhyd.2005.10.003 K. Williamson, L. Semprini, G. Rorrer, and J. McGuire. Integration of Chemical Engineering, Environmental Engineering, and Bioengineering to Facilitate Research and Education in Nanotechnology, Biotechnology, and Sustainability. Water Environment Research 78(6):555-556 (2006). Kim, Y. and L. Semprini, Cometabolic transformation of cis-1,2-dichloroethylene and cis-1,2-dichloroethylene epoxide by a butane grown mixed culture, Water Science and Technology, 52(8) 113-121 (2005). Niemet, Michael R. and L. Semprini, Column Studies of Anaerobic Carbon Tetrachloride Biotransformation with Hanford Aquifer Material, Ground Water Monitoring and Remediation, 25, 82-92 (2005). Davis, B. M., J.D. Istok, and L. Semprini, Numerical Simulations of Radon as an In Situ Partitioning Tracer for Quantifying NAPL Contamination Using Push-Pull Tests, Journal of Contaminant Hydrology, 78, 87-103 (2005). Field, J. A., R. Reed, J. D. Istok, L. Semprini, P. Bennett, and T. E. Buscheck, Trichlorofluoroethene: A Reactive Tracer for Evaluating Reductive Dechlorination in Large-Diameter Permeable Columns,Ground Water Monitoring and Remediation, 25(2) 68-77 (2005). Yu, S., M. Dolan and L. Semprini, Kinetics and Inhibition of Reductive Dechlorination of Chlorinated Ethylenes by Two Different Mixed Cultures, Environ. Sci. Technol., 39, 195-205 (2005). M.F.Azizian, J.D. Istok, and L. Semprini, Push-Pull test evaluation of the in situ aerobic cometabolism of chlorinated ethenes by toluene-utilizing microorganisms, Water Science and Technology, Vol. 52 no. 7, 35-40 (2005). J. A.C. Barth, A. Kappler, M. Piepenbrink, C. Werth, S. Regenspurg, L. Semprini, G. F. Slater, C. Schüth, P. Grathwohl . New Demands in Biogeochemical Gradient Research on Microbes, Measurements and Modeling. EOS 86(44):432(2005). E. Ennis, R. Reed, M. Dolan, L. Semprini, J. Istok, and J. Field, Reductive Dechlorination of the Vinyl Chloride Surrogate Chlorofluoroethene in TCE-Contaminated Groundwater, Environ. Sci. Technol., Vol. 39, 6777-6785 (2005). DOI link: http://dx.doi.org/10.1021/es048640f Connon, S., A. Tovanabootr, M. Dolan, K. Vergin, S. Giovannoni, and L. Semprini, Bacterial Community Composition Determined by Culture-Independent and -Dependent Methods During Propane-Stimulated Bioremediation in Trichloroethene Contaminated Groundwater, Environmental Microbiology, 7 (2), 165–178 (2005). List of All Journal Articles

Environmental Engineering, Undergraduate

Environmental Engineering,
Graduate

Subsurface Biosphere Initiative

Western Region Hazardous Substance Research Center

Earth's Subsurface Biosphere (ESB) IGERT Program

Lewis Semprini

Distinguished Professor of Environmental Engineering
Chair of the Executive Committee, Subsurface Biosphere Initiative

Curriculum Vita
Journal Articles
Conference Proceedings
Research Reports
Other Publications
Online Tutorials
Contact

Courses Taught

ENVE 554 Groundwater Remediation (link to related online tutorials)
ENVE 535 Hazardous Waste Remediation
ENVE431/531 Fate and Transport of Organic Chemicals in the Environment

Research Projects

Aerobic (Co)Metabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols [Slide]

Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity [Slide]

Global Transcriptional Responses in Nitrogen Cycling and Nutrient Removal Processes [Slide]

Provost's Initiative on Subsurface Biosphere Education and Research [Description]

Recent Publications

Yu, S. and, L. Semprini, Enhanced reductive dechlorination of PCE DNAPL with TBOS as a slow-release electron donor, J. Hazard. Mater. 167 (2009) 97–104. DOI link: http://dx.doi.org/10.1016/j.jhazmat.2008.12.087

Radniecki, Tyler S., Lewis Semprini, and Mark E. Dolan, Expression of merA, amoA and hao in Continuously Cultured Nitrosomonas europaea Cells Exposed to Zinc Chloride Additions, Biotechnology and Bioengineering, 102 (2) (2009) 546-553. DOI link: http://dx.doi.org/10.1002/bit.22069

Semprini, L., Mark E. Dolan, Gary D. Hopkins, and Perry L. McCarty, Bioaugmentation with butane-utilizing microorganisms to promote in situ cometabolic treatment of 1,1,1-trichloroethane and 1,1-dichloroethene, Journal of Contaminant Hydrology 103, (2009) 157-167. DOI link: http://dx.doi.org/10.1016/j.jconhyd.2008.10.005

Azizian, M.F., S. Behrens, A. Sabalowsky, M.E. Dolan, A.M. Spormann, and L. Semprini, Continuous-flow column study of reductive dehalogenation of PCE upon bioaugmentation with the Evanite enrichment culture, Journal of Contaminant Hydrology 100 (2008) 11–21. DOI link: http://dx.doi.org/10.1016/j.jconhyd.2008.04.006

Behrens, Sebastian, Mohammad F. Azizian, Paul J. McMurdie, Andrew Sabalowsky, Mark E. Dolan, Lew Semprini, and Alfred M. Spormann, Monitoring Abundance and Expression of "Dehalococcoides" Species Chloroethene-Reductive Dehalogenases in a Tetrachloroethene-Dechlorinating Flow Column, Appl. Envir. Microbiol. 2008 74: 5695-5703. DOI link: http://dx.doi.org/10.1128/AEM.00926-08

Kim, Y., J. D. Istok, L. Semprini, Single-well, gas-sparging tests for evaluating the in situ aerobic cometabolism of cis-1,2-dichloroethene and trichloroethene, Chemosphere 71 (2008) 1654–1664. DOI link: http://dx.doi.org/10.1016/j.chemosphere.2008.01.021

Semprini, L., M. E. Dolan, M. A. Mathias, G. D. Hopkins and P. L. McCarty, Bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane, European Journal of Soil Biology 43, (5-6), 322-327 (2007) (In situ Bioremediation, C/Tur Third European Bioremediation Conference). DOI link: http://dx.doi.org/10.1016/j.ejsobi.2007.03.006

A.E. Taylor, M.E. Dolan, P.J. Bottomley, and L. Semprini. Utilization of Fluoroethene as a Surrogate for Aerobic Vinyl Chloride Degradation. Environ. Sci. Technol., 41 (18) 6378–6383 (2007). DOI link: http://dx.doi.org/10.1021/es0701255

Yu, S. and L. Semprini. Enhanced Reductive Dechlorination of PCE DNAPL with TBOS as a Slow-Release Substrate. In review, Journal of Hazardous Waste.

Semprini, L., M. E. Dolan, M. A. Mathias, G. D. Hopkins and P. L. McCarty, Laboratory, field and modeling studies of bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane, Advances in Water Resources, 30 1528-1546 (2007). DOI link: http://dx.doi.org/10.1016/j.advwatres.2006.05.017

Azizian, M., J. D. Istok, L. Semprini, Evaluation of the in-situ aerobic cometabolism of chlorinated ethenes by toluene-utilizing microorganisms using push–pull tests, Journal of Contaminant Hydrology, 90 105–124 (2007). DOI link: http://dx.doi.org/10.1016/j.jconhyd.2006.09.015

Kim, Y., Istok, J. and L. Semprini, Push–pull tests evaluating in situ aerobic cometabolism of ethylene, propylene, and cis-1,2-dichloroethylene, Journal of Contaminant Hydrology, 82:165-181 (2006). DOI link: http://dx.doi.org/10.1016/j.jconhyd.2005.10.003

K. Williamson, L. Semprini, G. Rorrer, and J. McGuire. Integration of Chemical Engineering, Environmental Engineering, and Bioengineering to Facilitate Research and Education in Nanotechnology, Biotechnology, and Sustainability. Water Environment Research 78(6):555-556 (2006).

Kim, Y. and L. Semprini, Cometabolic transformation of cis-1,2-dichloroethylene and cis-1,2-dichloroethylene epoxide by a butane grown mixed culture, Water Science and Technology, 52(8) 113-121 (2005).

Niemet, Michael R. and L. Semprini, Column Studies of Anaerobic Carbon Tetrachloride Biotransformation with Hanford Aquifer Material, Ground Water Monitoring and Remediation, 25, 82-92 (2005).

Davis, B. M., J.D. Istok, and L. Semprini, Numerical Simulations of Radon as an In Situ Partitioning Tracer for Quantifying NAPL Contamination Using Push-Pull Tests, Journal of Contaminant Hydrology, 78, 87-103 (2005).

Field, J. A., R. Reed, J. D. Istok, L. Semprini, P. Bennett, and T. E. Buscheck, Trichlorofluoroethene: A Reactive Tracer for Evaluating Reductive Dechlorination in Large-Diameter Permeable Columns,Ground Water Monitoring and Remediation, 25(2) 68-77 (2005).

Yu, S., M. Dolan and L. Semprini, Kinetics and Inhibition of Reductive Dechlorination of Chlorinated Ethylenes by Two Different Mixed Cultures, Environ. Sci. Technol., 39, 195-205 (2005).

M.F.Azizian, J.D. Istok, and L. Semprini, Push-Pull test evaluation of the in situ aerobic cometabolism of chlorinated ethenes by toluene-utilizing microorganisms, Water Science and Technology, Vol. 52 no. 7, 35-40 (2005).

J. A.C. Barth, A. Kappler, M. Piepenbrink, C. Werth, S. Regenspurg, L. Semprini, G. F. Slater, C. Schüth, P. Grathwohl . New Demands in Biogeochemical Gradient Research on Microbes, Measurements and Modeling. EOS 86(44):432(2005).

E. Ennis, R. Reed, M. Dolan, L. Semprini, J. Istok, and J. Field, Reductive Dechlorination of the Vinyl Chloride Surrogate Chlorofluoroethene in TCE-Contaminated Groundwater, Environ. Sci. Technol., Vol. 39, 6777-6785 (2005). DOI link: http://dx.doi.org/10.1021/es048640f

Connon, S., A. Tovanabootr, M. Dolan, K. Vergin, S. Giovannoni, and L. Semprini, Bacterial Community Composition Determined by Culture-Independent and -Dependent Methods During Propane-Stimulated Bioremediation in Trichloroethene Contaminated Groundwater, Environmental Microbiology, 7 (2), 165–178 (2005).

List of All Journal Articles