Lewis Semprini

Environmental Engineering, Undergraduate Environmental Engineering, Graduate Subsurface Biosphere Initiative Western Region Hazardous Substance Research Center Earth's Subsurface Biosphere (ESB) IGERT Program	Lewis Semprini Distinguished Professor of Environmental Engineering Chair of the Executive Committee, Subsurface Biosphere Initiative Curriculum Vita Journal Articles Conference Proceedings Research Reports Other Publications Online Tutorials Contact
Courses Taught ENVE 554 Groundwater Remediation (link to related online tutorials) ENVE 535 Hazardous Waste Remediation ENVE431/531 Fate and Transport of Organic Chemicals in the Environment Research Projects Aerobic (Co)Metabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols [Slide] Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity [Slide] Global Transcriptional Responses in Nitrogen Cycling and Nutrient Removal Processes [Slide] Provost's Initiative on Subsurface Biosphere Education and Research [Description] Recent Publications Azizian, M.F., S. Behrens, A. Sabalowsky, M.E. Dolan, A.M. Spormann, and L. Semprini, Continuous-flow column study of reductive dehalogenation of PCE upon bioaugmentation with the Evanite enrichment culture, Journal of Contaminant Hydrology 100 (2008) 11–21. Kim, Y., J. D. Istok, L. Semprini, Single-well, gas-sparging tests for evaluating the in situ aerobic cometabolism of cis-1,2-dichloroethene and trichloroethene, Chemosphere 71 (2008) 1654–1664. Semprini, L., M. E. Dolan, M. A. Mathias, G. D. Hopkins and P. L. McCarty, Bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane, European Journal of Soil Biology 43, (5-6), 322-327 (2007) (In situ Bioremediation, C/Tur Third European Bioremediation Conference). DOI link: http://dx.doi.org/10.1016/j.ejsobi.2007.03.006 S. Behrens, M. Azizian, J. McMurdie, A. Sabalowsky, M.E. Dolan, L. Semprini, and A.M. Spormann. Spatial Distribution and Activity of Dehalococcoides Subpopulations Inhabiting a PCE-Dechlorinating Continuous Flow Reactor. Submitted to Applied and Environmental Microbiology. M. Azizian, S. Behrens, A. Sabalowsky, P. Ruiz-Haas, J. D. Ingle, Jr., M. Dolan, A. Spormann, and L. Semprini Continuous-Flow Bioreactor Study of Reductive Dehalogenation of PCE Upon Bioaugmenation with Evanite Enrichment Culture. Submitted to Applied and Environmental Microbiology. A.E. Taylor, M.E. Dolan, P.J. Bottomley, and L. Semprini. Utilization of Fluoroethene as a Surrogate for Aerobic Vinyl Chloride Degradation. In press, Environ. Sci. Technol. Azizian, M., J. D. Istok, L. Semprini, Evaluation of the in-situ aerobic cometabolism of chlorinated ethenes by toluene-utilizing microorganisms using push–pull tests, Journal of Contaminant Hydrology, 90 105–124 (2007). Semprini, L., M. E. Dolan, M. A. Mathias, G. D. Hopkins and P. L. McCarty, Laboratory, field and modeling studies of bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane, Advances in Water Resources, 30 1528-1546 (2007). Kim, Y., Istok, J. and L. Semprini, Push–pull tests evaluating in situ aerobic cometabolism of ethylene, propylene, and cis-1,2-dichloroethylene, Journal of Contaminant Hydrology, 82:165-181 (2006). K. Williamson, L. Semprini, G. Rorrer, and J. McGuire. Integration of Chemical Engineering, Environmental Engineering, and Bioengineering to Facilitate Research and Education in Nanotechnology, Biotechnology, and Sustainability. Water Environment Research 78(6):555-556 (2006). Kim, Y. and L. Semprini, Cometabolic transformation of cis-1,2-dichloroethylene and cis-1,2-dichloroethylene epoxide by a butane grown mixed culture, Water Science and Technology, 52(8) 113-121 (2005). Niemet, Michael R. and L. Semprini, Column Studies of Anaerobic Carbon Tetrachloride Biotransformation with Hanford Aquifer Material, Ground Water Monitoring and Remediation, 25, 82-92 (2005). Davis, B. M., J.D. Istok, and L. Semprini, Numerical Simulations of Radon as an In Situ Partitioning Tracer for Quantifying NAPL Contamination Using Push-Pull Tests, Journal of Contaminant Hydrology, 78, 87-103 (2005). Field, J. A., R. Reed, J. D. Istok, L. Semprini, P. Bennett, and T. E. Buscheck, Trichlorofluoroethene: A Reactive Tracer for Evaluating Reductive Dechlorination in Large-Diameter Permeable Columns,Ground Water Monitoring and Remediation, 25(2) 68-77 (2005). Yu, S., M. Dolan and L. Semprini, Kinetics and Inhibition of Reductive Dechlorination of Chlorinated Ethylenes by Two Different Mixed Cultures, Environ. Sci. Technol., 39, 195-205 (2005). M.F.Azizian, J.D. Istok, and L. Semprini, Push-Pull test evaluation of the in situ aerobic cometabolism of chlorinated ethenes by toluene-utilizing microorganisms, Water Science and Technology, Vol. 52 no. 7, 35-40 (2005). J. A.C. Barth, A. Kappler, M. Piepenbrink, C. Werth, S. Regenspurg, L. Semprini, G. F. Slater, C. Schüth, P. Grathwohl . New Demands in Biogeochemical Gradient Research on Microbes, Measurements and Modeling. EOS 86(44):432(2005). E. Ennis, R. Reed, M. Dolan, L. Semprini, J. Istok, and J. Field, Reductive Dechlorination of the Vinyl Chloride Surrogate Chlorofluoroethene in TCE-Contaminated Groundwater, Environ. Sci. Technol., Vol. 39, 6777-6785 (2005). Connon, S., A. Tovanabootr, M. Dolan, K. Vergin, S. Giovannoni, and L. Semprini, Bacterial Community Composition Determined by Culture-Independent and -Dependent Methods During Propane-Stimulated Bioremediation in Trichloroethene Contaminated Groundwater, Environmental Microbiology, 7 (2), 165–178 (2005). List of All Journal Articles

Environmental Engineering, Undergraduate

Environmental Engineering,
Graduate

Subsurface Biosphere Initiative

Western Region Hazardous Substance Research Center

Earth's Subsurface Biosphere (ESB) IGERT Program

Lewis Semprini

Distinguished Professor of Environmental Engineering
Chair of the Executive Committee, Subsurface Biosphere Initiative

Curriculum Vita
Journal Articles
Conference Proceedings
Research Reports
Other Publications
Online Tutorials
Contact

Courses Taught

ENVE 554 Groundwater Remediation (link to related online tutorials)
ENVE 535 Hazardous Waste Remediation
ENVE431/531 Fate and Transport of Organic Chemicals in the Environment

Research Projects

Aerobic (Co)Metabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols [Slide]

Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity [Slide]

Global Transcriptional Responses in Nitrogen Cycling and Nutrient Removal Processes [Slide]

Provost's Initiative on Subsurface Biosphere Education and Research [Description]

Recent Publications

Azizian, M.F., S. Behrens, A. Sabalowsky, M.E. Dolan, A.M. Spormann, and L. Semprini, Continuous-flow column study of reductive dehalogenation of PCE upon bioaugmentation with the Evanite enrichment culture, Journal of Contaminant Hydrology 100 (2008) 11–21.

Kim, Y., J. D. Istok, L. Semprini, Single-well, gas-sparging tests for evaluating the in situ aerobic cometabolism of cis-1,2-dichloroethene and trichloroethene, Chemosphere 71 (2008) 1654–1664.

Semprini, L., M. E. Dolan, M. A. Mathias, G. D. Hopkins and P. L. McCarty, Bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane, European Journal of Soil Biology 43, (5-6), 322-327 (2007) (In situ Bioremediation, C/Tur Third European Bioremediation Conference). DOI link: http://dx.doi.org/10.1016/j.ejsobi.2007.03.006

S. Behrens, M. Azizian, J. McMurdie, A. Sabalowsky, M.E. Dolan, L. Semprini, and A.M. Spormann. Spatial Distribution and Activity of Dehalococcoides Subpopulations Inhabiting a PCE-Dechlorinating Continuous Flow Reactor. Submitted to Applied and Environmental Microbiology.

M. Azizian, S. Behrens, A. Sabalowsky, P. Ruiz-Haas, J. D. Ingle, Jr., M. Dolan, A. Spormann, and L. Semprini Continuous-Flow Bioreactor Study of Reductive Dehalogenation of PCE Upon Bioaugmenation with Evanite Enrichment Culture. Submitted to Applied and Environmental Microbiology.

A.E. Taylor, M.E. Dolan, P.J. Bottomley, and L. Semprini. Utilization of Fluoroethene as a Surrogate for Aerobic Vinyl Chloride Degradation. In press, Environ. Sci. Technol.

Azizian, M., J. D. Istok, L. Semprini, Evaluation of the in-situ aerobic cometabolism of chlorinated ethenes by toluene-utilizing microorganisms using push–pull tests, Journal of Contaminant Hydrology, 90 105–124 (2007).

Semprini, L., M. E. Dolan, M. A. Mathias, G. D. Hopkins and P. L. McCarty, Laboratory, field and modeling studies of bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane, Advances in Water Resources, 30 1528-1546 (2007).

Kim, Y., Istok, J. and L. Semprini, Push–pull tests evaluating in situ aerobic cometabolism of ethylene, propylene, and cis-1,2-dichloroethylene, Journal of Contaminant Hydrology, 82:165-181 (2006).

K. Williamson, L. Semprini, G. Rorrer, and J. McGuire. Integration of Chemical Engineering, Environmental Engineering, and Bioengineering to Facilitate Research and Education in Nanotechnology, Biotechnology, and Sustainability. Water Environment Research 78(6):555-556 (2006).

Kim, Y. and L. Semprini, Cometabolic transformation of cis-1,2-dichloroethylene and cis-1,2-dichloroethylene epoxide by a butane grown mixed culture, Water Science and Technology, 52(8) 113-121 (2005).

Niemet, Michael R. and L. Semprini, Column Studies of Anaerobic Carbon Tetrachloride Biotransformation with Hanford Aquifer Material, Ground Water Monitoring and Remediation, 25, 82-92 (2005).

Davis, B. M., J.D. Istok, and L. Semprini, Numerical Simulations of Radon as an In Situ Partitioning Tracer for Quantifying NAPL Contamination Using Push-Pull Tests, Journal of Contaminant Hydrology, 78, 87-103 (2005).

Field, J. A., R. Reed, J. D. Istok, L. Semprini, P. Bennett, and T. E. Buscheck, Trichlorofluoroethene: A Reactive Tracer for Evaluating Reductive Dechlorination in Large-Diameter Permeable Columns,Ground Water Monitoring and Remediation, 25(2) 68-77 (2005).

Yu, S., M. Dolan and L. Semprini, Kinetics and Inhibition of Reductive Dechlorination of Chlorinated Ethylenes by Two Different Mixed Cultures, Environ. Sci. Technol., 39, 195-205 (2005).

M.F.Azizian, J.D. Istok, and L. Semprini, Push-Pull test evaluation of the in situ aerobic cometabolism of chlorinated ethenes by toluene-utilizing microorganisms, Water Science and Technology, Vol. 52 no. 7, 35-40 (2005).

J. A.C. Barth, A. Kappler, M. Piepenbrink, C. Werth, S. Regenspurg, L. Semprini, G. F. Slater, C. Schüth, P. Grathwohl . New Demands in Biogeochemical Gradient Research on Microbes, Measurements and Modeling. EOS 86(44):432(2005).

E. Ennis, R. Reed, M. Dolan, L. Semprini, J. Istok, and J. Field, Reductive Dechlorination of the Vinyl Chloride Surrogate Chlorofluoroethene in TCE-Contaminated Groundwater, Environ. Sci. Technol., Vol. 39, 6777-6785 (2005).

Connon, S., A. Tovanabootr, M. Dolan, K. Vergin, S. Giovannoni, and L. Semprini, Bacterial Community Composition Determined by Culture-Independent and -Dependent Methods During Propane-Stimulated Bioremediation in Trichloroethene Contaminated Groundwater, Environmental Microbiology, 7 (2), 165–178 (2005).

List of All Journal Articles